Inhalation chambers are commonly used to deliver aerosol drugs to cats with lower airway disease. Manufacturers recommend different cleaning procedures to minimize bacteria buildup in these spacer devices, but no studies have been performed to evaluate their effectiveness. The investigators in this study placed standardized inoculations of Pseudomonas aeruginosa into spacer devices from 2 different manufacturers. Devices were then cleaned according to manufacturer recommendations. Chambers were air dried for 24 hours, and samples were obtained from 3 sites and submitted for bacterial culture testing. No bacterial contamination was detected in any of the devices tested. The authors concluded that successful bacterial decontamination occurs when inhalation chambers are cleaned following manufacturer instructions.