Lissamine Green Staining for Tear Film Deficiency Evaluation

Anja Welihozkiy, DVM, DACVO, BluePearl Veterinary Partners, Sarasota, Florida

ArticleLast Updated April 20213 min read
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In the Literature

Smith SM, Holt E, Aguirre GD. Conjunctival staining with lissamine green as a predictor of tear film deficiency in dogs. Vet Ophthalmol. 2020;23(4):624-631.


The Research …

Canine tear film deficiencies are commonly diagnosed in veterinary medicine and include both quantitative (keratoconjunctivitis sicca [KCS]) and qualitative deficiencies.1 Qualitative tear film deficiencies result from abnormal mucin or lipid distribution/production of the precorneal tear film and cause tear film instability with increased tear evaporation and poor tear coverage of the ocular surface. Both quantitative and qualitative deficiencies can cause ocular irritation, as evidenced by conjunctival hyperemia; discharge; blepharospasm due to discomfort; palpebral, conjunctival, and corneal inflammation; and, in chronic cases, corneal pigmentation.

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