Trixie, a 2-year-old, 44-lb (20-kg) spayed German shepherd crossbreed, was presented for an ≈3-month history of chronic, marked weight loss and small bowel diarrhea. Stool was voluminous, pale in color, and soft and unformed in consistency. Her owner reported that Trixie had a good appetite and appeared to be healthy otherwise. Trial treatment with a hypoallergenic and novel protein diet for 3 weeks did not ameliorate the diarrhea or weight loss.

On physical examination, Trixie was bright, alert, and responsive. Vital signs were within normal limits. Her BCS was 2/9 and she had marked muscle wasting (Figure). Abdominal palpation was normal, and soft, yellow feces was detected during rectal examination; flatulence was also noted. The rest of the examination was within normal limits.

Differential diagnoses included intestinal parasitism, chronic enteropathy (eg, food-responsive enteropathy, antibiotic-responsive enteropathy, immunosuppressant-responsive enteropathy), protein-losing enteropathy, juvenile neoplasia, chronic intussusception, chronic foreign body, hypoadrenocorticism (ie, Addison’s disease), chronic kidney disease, chronic liver disease, and infection with Pythium spp, which is endemic in Florida.¹

CBC, serum chemistry profile, and urinalysis results were within normal limits. Fecal flotation results were negative.

Because of Trixie’s dramatic weight loss, abdominal radiography and ultrasonography were completed on the day of presentation. Radiographs were unremarkable but revealed mild loss of serosal detail, presumably secondary to patient emaciation. Ultrasound images revealed no mural thickening, abdominal mass, lymphadenopathy, or other abnormality. Adrenal glands were slightly decreased in size.

After Trixie was fasted for 12 hours, serum cobalamin (ie, vitamin B₁₂), folate, canine trypsin-like immunoreactivity (cTLI), and baseline cortisol levels were obtained (Table). The results demonstrated a decreased cTLI, which was diagnostic for exocrine pancreatic insufficiency. Cobalamin was also decreased, which was consistent with ileal pathology. Baseline cortisol was increased, ruling out hypoadrenocorticism.² 

Trixie was initially started on pancreatic enzyme replacement powder at 1 tsp/22 lb (10 kg) of body weight mixed with food.³ She was also given 1 cyanocobalamin tablet daily (1 mg PO every 24 hours is recommended for dogs weighing >44 lb [20 kg]).⁴ Her owner was instructed to closely monitor Trixie’s stool for improvement in consistency, frequency, and volume and to return to the clinic every 2 weeks for assessment and monitoring for weight gain. Lifelong treatment with enzyme replacement therapy and cyanocobalamin is recommended for exocrine pancreatic insufficiency. Trixie was also empirically dewormed with fenbendazole (50 mg/kg/day for 5 days).⁵

Trixie was returned for a recheck examination 2 weeks after presentation. She was rapidly gaining weight, and her stool had improved in quality but was still soft; however, she had also started periodically vomiting daily. Tylosin (25 mg/kg every 12 hours) was given because of her history of low cobalamin in conjunction with the high prevalence of dysbiosis and antibiotic-responsive enteropathy (formerly called small intestinal bacterial overgrowth) associated with exocrine pancreatic insufficiency (EPI).^6,7 Dysbiosis was most likely associated with changes in motility, lack of bacteriostatic pancreatic juices, and altered immune function.⁶ At the next recheck examination, the owner reported that Trixie was thought to be completely back to normal (ie, prior to the development of clinical signs). There were no GI signs, her BCS was 4/9 and expected to continue to improve, and her weight had increased to 57 lb (26 kg). Over the next few months, her BCS returned to normal (ie, 5/9) and her weight increased to 66 lb (30 kg); pancreatic enzyme replacement therapy was tapered to a lower dose. Tylosin was stopped without recurrence of signs ≈6 weeks after diagnosis. Cobalamin supplementation was continued, and Trixie was transitioned to a primary care veterinarian.