Quiz: PCR for the General Practitioner

Kyle Webb, DVM, DACVP, ANTECH Diagnostics, Orlando, FL

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Quiz: PCR for the General Practitioner

Polymerase chain reaction (PCR) uses amplification to detect infectious nucleic acids or those associated with disease processes, often as a primary or adjunct molecular diagnostic tool.

PCR has many applications in both human and veterinary medicine, but the most practical for the general veterinary practitioner are diagnosis of certain infectious diseases and detection of lymphoma. PCR for antigen receptor rearrangement (PARR), for example, assesses lymphocyte clonality, which can be helpful in cases where cytology is equivocal.

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Quiz: PCR for the General Practitioner

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What does PCR amplify?

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Quiz: PCR for the General Practitioner
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Which of the following infectious agents does not need to undergo reverse transcription before it can be amplified by PCR?

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Quiz: PCR for the General Practitioner
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When is the best time to collect a sample for PCR detection of infectious disease?

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A positive PCR result indicates active infection.

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PARR is more sensitive for diagnosis of lymphoma in dogs than in cats. 

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Which of the following is an indication for the use of PARR?

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Which of the following agents can cause a false-positive PARR result?

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Quiz: PCR for the General Practitioner
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Quiz: PCR for the General Practitioner

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